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Microfluidic thread‐based electrode system to detect glucose and acetylthiocholine
Author(s) -
Gaines Michelle,
GonzalezGuerrero Maria Jose,
Uchida Kathryn,
Gomez Frank A.
Publication year - 2018
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800348
Subject(s) - glucose oxidase , potassium ferricyanide , acetylthiocholine , electrode , silver chloride , chemistry , cyclic voltammetry , chromatography , materials science , nuclear chemistry , electrochemistry , inorganic chemistry , organic chemistry , aché , acetylcholinesterase , enzyme
A reusable and simple to fabricate electrochemical sensor for the detection of glucose and acetylthiocholine using thread‐based electrodes and nylon thread is described. The fabrication of the device consisted of two steps. First, three nylon‐based electrodes (reference, working, and counter) were painted with one layer of conductive inks (silver and carbon ink, or silver/silver chloride ink). The electrodes were taped onto parafilm, and a piece of white nylon thread was wrapped around each electrode connecting the three electrodes. For the glucose system, a PBS solution containing glucose oxidase (GOx) (10 mg/mL), and potassium ferricyanide (K 3 [Fe(CN) 6 ]) (10 mg/mL) as mediator, was dried onto the thread, and increasing concentrations of glucose (0–15 mM) was added to the thread and measured by cyclic voltammetry (CV). The current output from the glucose oxidation was proportional to the concentration of glucose. For the second system, a solution of acetylcholinesterase (AChE) (0.08 U/mL) in PBS was added to the nylon thread, and increasing concentrations of acetylthiocholine (ATC) (0–9.84 mg/mL) was added and measured by CV. The current output from the oxidation of thiocholine (produced by AChE reacting with ATC) was proportional to the concentrations of ATC added to the thread. From both systems, a graph of current output versus substrate concentration was produced and fitted with a linear regression line that gave R 2 values of 0.985 (GO X /glucose) and 0.995 (AChE/ATC).