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A fluorescence toolbox: A review of investigation of electrophoretic separations, process, and interfaces
Author(s) -
Thompson S.,
Pappas Dimitri
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800310
Subject(s) - capillary electrophoresis , fluorescence , electrophoresis , electrokinetic phenomena , fluorescence spectroscopy , fluorescence correlation spectroscopy , fluorescence recovery after photobleaching , chromatography , chemistry , laser induced fluorescence , fluorescence microscope , analytical chemistry (journal) , photobleaching , micellar electrokinetic chromatography , fluorescence anisotropy , materials science , nanotechnology , optics , physics
This review focuses on fluorescence spectroscopy techniques for the investigation of electrophoretic separations. Fluorescence has been used as a sensitive detector for capillary, gel, and microchip electrophoresis for decades. However, advanced fluorescence methods can be used to study transport, interfacial phenomena, intermolecular and affinity interactions, and other processes that occur during separation. This so‐called spectroscopic toolkit can be implemented to understand fundamental behavior in electrophoresis and electrokinetic chromatography. Techniques such as fluorescence recovery after photobleaching, fluorescence correlation spectroscopy, and fluorescence anisotropy are discussed in relation to electrophoretic separations. Newer methods such as super‐resolution microscope are also introduced.