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Synergistic enantioseparation systems with either cyclodextrins or cyclofructans and L‐alanine Tert butyl ester lactate
Author(s) -
Nicolaou Athina G.,
Mavroudi Maria C.,
Stavrou Ioannis J.,
Weatherly Choyce A.,
KapnissiChristodoulou Constantina P.
Publication year - 2019
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800257
Subject(s) - chemistry , enantiomer , analyte , chromatography , fexofenadine , cyclodextrin , ionic liquid , combinatorial chemistry , chiral derivatizing agent , resolution (logic) , alanine , amino acid , organic chemistry , chiral column chromatography , catalysis , medicine , biochemistry , artificial intelligence , computer science , pharmacology
The combined use of chiral ionic liquids (CILs) and conventional chiral selectors (CSs) in CE, to establish a synergistic system, has proven to be an effective approach for the separation of enantiomeric pairs. In this study, a new CE method was developed, employing a binary system of a CS, either a cyclodextrin (CD) or a cyclofructan (CF), and a chiral amino acid ester‐based ionic liquid (AAIL), for the chiral separation of four basic, acidic and zwitterionic drug compounds. In particular, the enantioseparation of two anticoagulants, warfarin (WAR) and coumachlor (COU), a non‐opioid analgesic, nefopam (NEF) and a third‐generation antihistamine, fexofenadine (FXD), was examined, by supporting the BGE with a CS and the chiral AAIL L‐alanine tert butyl ester lactate (L‐AlaC 4 Lac). Parameters, such as the type of the CS, the concentration of both the CS and L‐AlaC 4 Lac, and the BGE pH, were methodically examined in order to optimize the chiral separation of each analyte. It was observed that, in some cases, the addition of the AAIL into the BGE improved both resolution (R s ) and efficiency (N) significantly. In other cases, the synergistic effect enabled baseline separation of analyte enantiomers, at a much lower concentration of the CS. Finally, after optimization of separation conditions, baseline separations (R s >1.5) of all four analytes were achieved in less than 5 min.