Premium
Development of green extraction processes for Nannochloropsis gaditana biomass valorization
Author(s) -
SánchezCamargo Andrea del Pilar,
Pleite Natalia,
Mendiola José Antonio,
Cifuentes Alejandro,
Herrero Miguel,
GilbertLópez Bienvenida,
Ibáñez Elena
Publication year - 2018
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800122
Subject(s) - chromatography , chemistry , chromatography detector , palmitoleic acid , high performance liquid chromatography , extraction (chemistry) , response surface methodology , biorefinery , phaeodactylum tricornutum , palmitic acid , fatty acid , organic chemistry , botany , raw material , biology , diatom
In the present work, the valorization of Nannochloropsis gaditana biomass is proposed within the concept of biorefinery. To this aim, high‐pressure homogenization (HPH) was used to break down the strong cell wall and supercritical fluid extraction (SFE) with pure CO 2 was applied as a first step to extract valuable compounds (such as non‐polar lipids and pigments). Extraction of the remaining residue for the recovery of bioactive compounds was studied by means of an experimental design based on response surface methodology (RSM) employing pressurized liquid extraction (PLE) with green solvents such as water and ethanol. Optimum extract was achieved with pure ethanol at 170°C for 20 min, providing an important antioxidant capacity (0.72 ± 0.03 mmol trolox eq g −1 extract). Complete chemical characterization of the optimum extract was carried out by using different chromatographic methods such as reverse‐phase high‐performance liquid chromatography with diode array detection (RP‐HPLC‐DAD), normal‐phase HPLC with evaporative light scattering detection (NP‐HPLC‐ELSD) and gas chromatography coupled to mass spectrometry detection (GC‐MS); carotenoids (e.g. violaxanthin), chlorophylls and polar lipids were the main compounds observed while palmitoleic, palmitic, myristic acids and the polyunsaturated eicosapentanoic (EPA) acid were the predominant fatty acids in all PLE extracts.