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Carbon molecular sieve based micro‐matrix‐solid‐phase dispersion for the extraction of polyphenols in pomegranate peel by UHPLC‐Q‐TOF/MS
Author(s) -
Du LiJing,
Huang JianPing,
Wang Bin,
Wang ChenHui,
Wang QiuYan,
Hu YuHan,
Yi Ling,
Cao Jun,
Peng LiQing,
Chen YuBo,
Zhang QiDong
Publication year - 2018
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800045
Subject(s) - chromatography , extraction (chemistry) , ellagic acid , dispersant , solid phase extraction , elution , chemistry , sieve (category theory) , matrix (chemical analysis) , gallic acid , polyphenol , sample preparation , dispersion (optics) , organic chemistry , physics , mathematics , combinatorics , optics , antioxidant
A rapid, simple, and efficient sample extraction method based on micro‐matrix‐solid‐phase dispersion (micro‐MSPD) was applied to the extraction of polyphenols from pomegranate peel. Five target analytes were determined by ultra‐HPLC coupled with Q‐TOF/MS. Carbon molecular sieve (CMS) was firstly used as dispersant to improve extraction efficiency in micro‐MSPD. The major micro‐MSPD parameters, such as type of dispersant, amount of dispersant, grinding time, and the type and the volume of elution solvents, were studied and optimized. Under optimized conditions, 26 mg of pomegranate peel was dispersed with 32.5 mg of CMS, the grinding time was selected as 90 s, the dispersed sample was eluted with 100 μL of methanol. Results showed that the proposed method was of good linearity for concentrations of analytes against their peak areas (coefficient of determination r 2 > 0.990), the LOD was as low as 3.2 ng/mL, and the spiking recoveries were between 88.1 and 106%. Satisfactory results were obtained for the extraction of gallic acid, punicalagin A, punicalagin B, catechin, and ellagic acid from pomegranate peel sample, which demonstrated nice reliability and high sensitivity of this approach.