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Use of Endoglycosidase H as a diagnostic tool for MAN1B1‐CDG patients
Author(s) -
Duvet Sandrine,
Mouajjah Dounia,
Péanne Romain,
Matthijs Gert,
Raymond Kimiyo,
Jaeken Jaak,
Morava Eva,
Foulquier François
Publication year - 2018
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201800020
Subject(s) - glycosylation , glycan , endoglycosidase h , mannosidase , mannose , glycomics , endoglycosidase , chemistry , biochemistry , glycoprotein , computational biology , biology , cell , golgi apparatus
Congenital disorders of glycosylation (CDG) are heterogeneous group of genetic protein and lipid glycosylation abnormalities. With some 33 reported patients, MAN1B1‐CDG belongs to the more frequent causes of CDG‐II. MAN1B1 encodes an α1,2‐mannosidase that removes the terminal mannose residue from the middle branch. Several methods have been proposed to characterize the glycosylation changes. In MAN1B1‐CDG, the abnormal accumulating N‐glycan structures are mostly absent or found in trace amounts in total human serum. To overcome this issue, in this study, we present a straightforward procedure based on the use of Endo‐β‐N‐acetylglucosaminidase H to easily diagnose MAN1B1‐CDG patients and mannosidase defects.