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Improved consistency in 2D gel electrophoresis: Sheep plasma as a test case
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201770041
Subject(s) - consistency (knowledge bases) , matching (statistics) , chromatography , cover (algebra) , plasma , electrophoresis , computer science , chemistry , artificial intelligence , mathematics , statistics , physics , quantum mechanics , engineering , mechanical engineering
Stuart Brown and Gillian Norris Electrophoresis 2017, 38 , 906–913. DOI: 10.1002/elps.201600433 The cover photo shows two sets of replicate gels run with sheep plasma samples in 2D electrophoresis using different methods (a New Zealand pastoral scene is in the background). Gel consistency and resolution can be a problem with plasma samples run on 2D gels and causes difficulty with spot matching and quantitative analysis. After experimenting with a wide range of parameters a new method was developed for sheep plasma involving conditions at the IPG strip electrodes. The method was tested by analysing the standards from two different DIGE experiments, comparing the old and new methods.