Premium
Back Cover: Studying the effect of microwave heating on the digestion process and identification of proteins
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201770026
Subject(s) - digestion (alchemy) , cover (algebra) , detection limit , limit (mathematics) , microwave , trypsin , computer science , chemistry , microwave irradiation , chromatography , analytical chemistry (journal) , computational biology , biology , biochemistry , mathematics , enzyme , telecommunications , engineering , mechanical engineering , mathematical analysis
Shobha Devi, Bo‐Hung Wu, Pei‐Yu Chu, Yue‐Pei Liu, Hsin‐Lin Wu, and Yen‐Peng Ho Electrophoresis 2017, 38 , 429–440. DOI: 10.1002/elps.201600392 The Back Cover shows the impact of microwave irradiation on the in‐solution digestion processes and the detection limit of proteins. Kinetic processes of many peptides produced through the trypsin digestion of various proteins under microwave heating at 50 °C revealed incomplete digestion. We examined the detection limits of individual proteins under microwave heating at 50 °C and at different time intervals (1, 5 and 30 min) using LC‐MS. We conclude that, if the peptides without missed cleavage dictate the detection limit, conventional digestion will lead to a better detection limit.