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Development of an electrophoretic method based on nanostructured materials for HbA1c determination
Author(s) -
Hamer Mariana,
Bassi Narella,
Agata Grela Denise
Publication year - 2018
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201700484
Subject(s) - absorbance , calibration curve , chromatography , analytical chemistry (journal) , materials science , electrophoresis , resolution (logic) , nanoparticle , chemistry , detection limit , nanotechnology , computer science , artificial intelligence
Glycosylated hemoglobin (HbA1c) detection is performed routinely in hospitals as it is the most widespread confirmatory diagnosis of diabetes mellitus. Here we present a novel CE method for measuring HbA1c by introducing silica nanoparticles (NPs) modified with a boronic acid derivative (sugar loadings of 51 ± 2 μg/mg) as pseudo‐stationary phase. Before the sample injection, SiO 2 NP─B(OH) 2 were introduced via pressure. Electrophoretic separation was explored through variation of the buffer pH and separation voltage, being the best separation, resolution and shorter separation time achieved with a 25 mM phosphate buffer pH 6.5. The calibration curve obtained was expressed as Area = 182.05% −1 × HbA1c − 377.02; R 2 = 0.9826, using a UV/VIS absorbance detector at 415 nm (diode array). No interferences were observed from carbamylated or acetylated hemoglobin and the method shows a noteworthy stability. A paired t‐test was applied to compare the developed CE method with a commercial HbA1c test and no significant variations have been observed at a 90% significance level.