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Polymethacrylate‐based monoliths as stationary phases for separation of biopolymers and immobilization of enzymes
Author(s) -
Martinović Tamara,
Josić Djuro
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201700255
Subject(s) - biomolecule , chromatography , nucleic acid , biopolymer , denaturation (fissile materials) , chemistry , chromatographic separation , polymer , separation method , stationary phase , nanotechnology , materials science , organic chemistry , high performance liquid chromatography , biochemistry , nuclear chemistry
The experiences in the production and application of polymethacrylate‐based monolithic supports, since their development almost thirty years ago, are presented. The main driving force for the development of new chromatographic supports was the necessity for the isolation and separation of physiologically active biopolymers and their use for therapeutic purposes. For this sake, a development of a method for fast separation, preventing denaturation and preserving their biological activity was necessary. Development of polysaccharide‐based supports, followed by the introduction of polymer‐based chromatographic media, is shortly described. This development was followed by the advances in monolithic media that are now used for both large‐ and small‐scale separation of biopolymers and nanoparticles. Finally, a short overview is given about the applications of monoliths for sample displacement chromatography, resulting in isolation of physiologically active biomolecules, such as proteins, protein complexes, and nucleic acid, as well as high‐throughput sample preparation for proteomic investigations.