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Fast separation of enantiomers by capillary electrophoresis using a combination of two capillaries with different internal diameters
Author(s) -
Šebestová Andrea,
Petr Jan
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201700213
Subject(s) - capillary electrophoresis , chromatography , enantiomer , chemistry , capillary action , analytical chemistry (journal) , free flow electrophoresis , sodium , electrophoresis , separation (statistics) , materials science , stereochemistry , polyacrylamide gel electrophoresis , biochemistry , gel electrophoresis of proteins , organic chemistry , machine learning , computer science , composite material , enzyme
Abstract The combination of capillaries with different internal diameters was used to accelerate the separation of enantiomers in capillary electrophoresis. Separation of R , S ‐1,1′‐binaphthalene‐2,2′‐diyl hydrogen phosphate using isopropyl derivative of cyclofructan 6 was studied as a model system. The best separation conditions included 500 mM sodium borate pH 9.5 with 60 mM concentration of the chiral selector. Separation lasted approx. 1.5 min using the combination of 50 and 100 μm id capillaries of 9.7 cm and 22.9 cm, respectively. It allowed approx. 12‐fold acceleration in comparison to the traditional long‐end separation mainly due to the higher electroosmotic flow generated in the connected capillaries.