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Synthesis of supermacroporous cryogel for bioreactors continuous starch hydrolysis
Author(s) -
Guilherme Ederson Paulo Xavier,
Oliveira Jocilane Pereira,
Carvalho Lorendane Millena,
Brandi Igor Viana,
Santos Sérgio Henrique Sousa,
Carvalho Gleidson Giordano Pinto,
Cota Junio,
Mara Aparecida de Carvalho Bruna
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201700208
Subject(s) - starch , hydrolysis , aspergillus oryzae , chemistry , maltose , chromatography , acrylamide , bioreactor , alpha amylase , amylase , monomer , enzyme , organic chemistry , polymer
A bioreactor was built by means of immobilizing alpha‐amylase from Aspergillus oryzae by encapsulation, through cryopolymerization of acrylamide monomers for the continuous starch hydrolysis. The starch hydrolysis was evaluated regarding pH, the concentration of immobilized amylase on cryogel, the concentration of starch solution and temperature. The maximum value for starch hydrolysis was achieved at pH 5.0, concentration of immobilized enzyme 111.44 mg amylase /g cryogel , concentration of starch solution 45 g/L and temperature of 35°C. The immobilized enzyme showed a conversion ratio ranging from 68.2 to 97.37%, depending on the pH and temperature employed. Thus, our results suggest that the alpha‐amylase from A. oryzae immobilized on cryogel monoliths represents a potential process for industrial production of maltose from starch hydrolysis.