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Evaluation of capillary zone electrophoresis for the quality control of complex biologic samples: Application to snake venoms
Author(s) -
Kpaibe André P.S.,
BenAmeur Randa,
Coussot Gaëlle,
Ladner Yoann,
Montels Jérôme,
Ake Michèle,
Perrin Catherine
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201700158
Subject(s) - capillary electrophoresis , venom , fingerprint (computing) , chromatography , snake venom , peptide , repeatability , electrophoresis , biochemical engineering , chemistry , biology , computational biology , computer science , biochemistry , artificial intelligence , engineering
Snake venoms constitute a very promising resource for the development of new medicines. They are mainly composed of very complex peptide and protein mixtures, which composition may vary significantly from batch to batch. This latter consideration is a challenge for routine quality control (QC) in the pharmaceutical industry. In this paper, we report the use of capillary zone electrophoresis for the development of an analytical fingerprint methodology to assess the quality of snake venoms. The analytical fingerprint concept is being widely used for the QC of herbal drugs but rarely for venoms QC so far. CZE was chosen for its intrinsic efficiency in the separation of protein and peptide mixtures. The analytical fingerprint methodology was first developed and evaluated for a particular snake venom, Lachesis muta . Optimal analysis conditions required the use of PDADMAC capillary coating to avoid protein and peptide adsorption. Same analytical conditions were then applied to other snake venom species. Different electrophoretic profiles were obtained for each venom. Excellent repeatability and intermediate precision was observed for each batch. Analysis of different batches of the same species revealed inherent qualitative and quantitative composition variations of the venoms between individuals.