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Migration time shift of analytes in micellar electrokinetic chromatography induced by stacking
Author(s) -
Dziomba Szymon,
Ciura Krzesimir,
Markuszewski Michał J.,
Wielgomas Bartosz
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201700061
Subject(s) - micellar electrokinetic chromatography , chromatography , isotachophoresis , analyte , chemistry , electrokinetic phenomena , ascorbic acid , stacking , matrix (chemical analysis) , analytical chemistry (journal) , detection limit , electrolyte , organic chemistry , food science , electrode
A significant shift of migration time of nonretained compounds (ascorbic acid and cysteine) in micellar electrokinetic chromatography was observed under variation of sample matrix composition. The shift was affected by borate buffer concentration in sample matrix, sample injection time, and pH of BGE (80 mM SDS, Tris/HCl). Surprisingly, longer migration time of analyte was recorded at higher pH of separation buffer. These observations were linked to transient isotachophoresis process. Computer simulation with Simul5 software was conducted to support this hypothesis. The manuscript documents rarely reported in the literature phenomenon of isotachophoresis in micellar electrokinetic chromatography. The analytical potential of described observations was also discussed.