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Development of the 19 X‐STR loci multiplex system and genetic analysis of a Zhejiang Han population in China
Author(s) -
Yang XingYi,
Wu WeiWei,
Chen LinLi,
Liu ChangHui,
Zhang XiaoFang,
Chen Ling,
Feng XingLin,
Wang HuiJun,
Liu Chao
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201670131
Subject(s) - multiplex , microbiology and biotechnology , locus (genetics) , multiplex polymerase chain reaction , biology , taq polymerase , population , chemistry , genetics , dna , analytical chemistry (journal) , polymerase chain reaction , chromatography , dna polymerase , gene , medicine , thermus aquaticus , environmental health
Electrophoresis 2016, 37 , 2260–2272. DOI: 10.1002/elps.201500540 The 19 X‐STRs multiplex system is a PCR‐based amplification kit that facilitates simultaneous amplification of 19 X‐chromosomal STR loci. In this study, the multiplex system was tested for detection sensitivity, DNA mixtures, inhibitor tolerance and species specificity. The final hold time was tested with 0 min, 10 min, 20 min, 30 min (recommended) 40 min 50 min and 60 min. As Taq polymerase has the tendency of adding an extra, non‐template nucleotide at the 3' ends of DNA strands during thermal cycling. Therefore, sufficient final extension time was needed to adenylate the fragments. 1ng 9947a performed well at all extension times above 30min, but the DXS10164 locus displayed small minus‐A peaks under the conditions of 0 min and 10 min. Shoulders were detected in the direct amplification samples at 0 min, 10 min, and occasionally at 20 min. The figure shows effects of shortening the final extension time after normal thermal cycling for 1ng 9947a. The black arrows indicate the minus‐A shoulder peaks for the DXS10164.