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On‐chip quantitative‐PCR using integrated real‐time detection by capillary electrophoresis
Author(s) -
Liu Yu,
Li Chen,
Li Zhi,
Chan Samuel D.,
Eto Daisuke,
Wu Warren,
Zhang Jian Ping,
Chien RingLing,
Wada Henry G.,
Greenstein Michael,
Satomura Shinji
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201670031
Subject(s) - capillary electrophoresis , amplicon , real time polymerase chain reaction , chromatography , microfluidics , polymerase chain reaction , lab on a chip , electrophoresis , chip , microbiology and biotechnology , chemistry , biology , computer science , nanotechnology , gene , materials science , genetics , telecommunications
Electrophoresis 2016, 37 , 545–552. DOI: 10.1002/elps.201500298 Quantitative polymerase chain reaction (qPCR) and capillary electrophoresis (CE) were integrated into a microfluidic chip that was designed to achieve real‐time amplicon sampling, separation, and quantitation without requiring various probes. A novel channel design and PCR‐CE synchronization scheme allows the overlapped execution of PCR and CE, minimizing the time required for CE analysis after each PCR cycle. The performance of the on‐chip qPCRCE method was demonstrated using model assay protocols to detect phiX174 bacteriophage and E. coli genomic DNA. By combining the separation capability of CE and the amplification power of PCR assay, this method is expected to find applications in awide variety of infectious disease detection and monitoring assays.