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Sequence requirements of oligonucleotide chiral selectors for the capillary electrophoresis resolution of low‐affinity DNA binders
Author(s) -
Tohala Luma,
Oukacine Farid,
Ravelet Corinne,
Peyrin Eric
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600516
Subject(s) - oligonucleotide , resolution (logic) , capillary electrophoresis , dna , random coil , analyte , enantiomer , selectivity , sequence (biology) , chromatography , chemistry , combinatorial chemistry , analytical chemistry (journal) , materials science , circular dichroism , stereochemistry , organic chemistry , biochemistry , artificial intelligence , computer science , catalysis
We recently reported that a great variety of DNA oligonucleotides (ONs) used as chiral selectors in partial‐filling capillary electrophoresis (CE) exhibited interesting enantioresolution properties toward low‐affinity DNA binders. Herein, the sequence prerequisites of ONs for the CE enantioseparation process were studied. First, the chiral resolution properties of a series of homopolymeric sequences (Poly‐dT) of different lengths (from 5 to 60‐mer) were investigated. It was shown that the size increase‐dependent random coil‐like conformation of Poly‐dT favorably acted on the apparent selectivity and resolution. The base‐unpairing state constituted also an important factor in the chiral resolution ability of ONs as the switch from the single‐stranded to double‐stranded structure was responsible for a significant decrease in the analyte selectivity range. Finally, the chemical diversity enhanced the enantioresolution ability of single‐stranded ONs. The present work could lay the foundation for the design of performant ON chiral selectors for the CE separation of weak DNA binder enantiomers.