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Potential serum biomarkers associated with mild and severe leptospirosis infection: A cohort study in the Malaysian population
Author(s) -
Tan Xue Ting,
Amran Fairuz binti,
Thayan Ravindran,
Ahmad Norazah,
Jaafar Roslinda,
Haron Rahimah,
Abdullah Rafidah,
bin Shamsuddin Sazwan Reezal,
Md. Riffin Nor Suhaila binti,
AbdulRahman Puteri Shafinaz
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600471
Subject(s) - leptospirosis , transthyretin , haptoglobin , proteome , western blot , acute phase protein , serum amyloid a , leptospira , medicine , immunology , blood proteins , cohort , apolipoprotein b , transferrin , population , biology , virology , bioinformatics , biochemistry , inflammation , cholesterol , gene , environmental health
Leptospirosis is an emerging zoonotic infectious disease in Malaysia. The symptoms of leptospirosis vary from mild nonspecific flu‐like illness to a severe condition which is usually associated with serious complication and fatality. To study the protein expression profile of mild and severe leptospirosis, 15 paired sera were collected from the patients who were mildly infected and following that progressed to severe stage. The proteome profiles of mild and severe cases were studied using 2DE analysis in combination with LC‐MS/MS. The expression of proteins that were significantly different and had a fold difference of at least 2 had been identified and then validated using Western blot. Our study demonstrated apolipoprotein A‐I (APOA‐I), serum amyloid A (SAA), transferrin (TF), haptoglobin (HP) and transthyretin (TTR) have significantly different expression between mild and severe leptospirosis. The Ingenuity Pathway Analysis software suggested the expression of these five proteins were modulated by acute phase response signaling pathway. Besides that, a functional network of lipid metabolism, molecular transport and small molecule biochemistry that interconnects these five proteins with interactomes also had been predicted by this software. In conclusion, this finding supports the potential of these five proteins to be the biomarkers for mild and severe human leptospirosis.