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Tiny protein detection using pressure through solid‐state nanopores
Author(s) -
Li Ji,
Hu Rui,
Li Xiaoqing,
Tong Xin,
Yu Dapeng,
Zhao Qing
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600410
Subject(s) - nanopore , solid state , protein detection , nanotechnology , materials science , chemistry
Solid‐state nanopore is a promising tool to detect proteins and its complexes. Small proteins (sub‐35 kDa) translocate very fast which could not be detected by normal patch‐clamp recording instrument due to low temporal resolution. We first introduce pressure into protein study and detection. The pressure‐derived force, combined with the voltage bias, makes very tiny protein (MW < 6.5 kDa) detection possible. Capture rate for Aprotinin is enhanced five times more than that in traditional voltage‐driven method by fine tuning of pressure and voltage. Temporal resolution of Aprotinin detection has improved by decreasing effective driving force. Moreover, we provide potential method to locate the equilibrium range for BSA movement in ionic solution by modulating driving pressure and retard voltage. Our study is of fundamental significance in nanopore research and provides unique platforms to study small proteins and other tiny biomolecules.