Premium
Phospholipid bilayer affinities and solvation characteristics by electrokinetic chromatography with a nanodisc pseudostationary phase
Author(s) -
Penny William M.,
Steele Harmen B.,
Ross J.B. Alexander,
Palmer Christopher P.
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600381
Subject(s) - solvation , chemistry , nanodisc , hydrogen bond , bilayer , phase (matter) , lipophilicity , lipid bilayer , crystallography , chromatography , organic chemistry , molecule , membrane , biochemistry
Phospholipid bilayer nanodiscs composed of 1,2‐dimyristoyl‐ sn ‐glycero‐3‐phosphocholine and synthetic maleic acid–styrene copolymer belts have been introduced as a pseudostationary phase (PSP) in electrokinetic chromatography and demonstrated good performance. The nanodiscs provide a suitable migration range and high theoretical plate counts. Using this nanodisc pseudostationary phase, the affinity of the bilayer structure for probe solutes was determined and characterized. Good correlation is observed between retention factors and octanol water partition coefficients for particular categories of solutes, but the general correlation is weak primarily because the nanodiscs show stronger affinity than octanol for hydrogen bond donors. This suggests that a more appropriate application of this technology is to measure and characterize interactions between solutes and lipid bilayers directly. Linear solvation energy relationship analysis of the nanodisc–solute interactions in this study demonstrates that the nanodiscs provide a solvation environment with low cohesivity and weak hydrogen bond donating ability, and provide relatively strong hydrogen bond acceptor strength.