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Evaluation of protein separation mechanism and pore size distribution in colloidal self‐assembled nanoparticle sieves for on‐chip protein sizing
Author(s) -
Azim Mohammad,
Malekpourkoupaei Ali,
Ye Wenmin,
Jemere Abebaw B.,
Harrison D. Jed
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600339
Subject(s) - particle size , colloid , nanoparticle , particle (ecology) , materials science , electrophoresis , chromatography , molecular sieve , chemical engineering , sizing , dispersion (optics) , chemistry , nanotechnology , adsorption , organic chemistry , oceanography , physics , optics , geology , engineering
The separation behavior of 6.5–66 kDa proteins in silica particle array‐based sieves formed by colloidal self‐assembly in microchips is reported across a pore size range of 22–103 nm. The protein separation and resolution improves markedly with decreasing pore size. The variation of electrophoretic mobility with molecular weight of SDS–protein complexes and with particle size was evaluated using the Ogston sieving equation for a random pore gel structure, and using the modified Giddings equation developed by Wirth for uniform pore structures. The Wirth/Giddings equation provides the best fit for estimation of molecular weight of proteins, and demonstrates that even though experimental evidence shows there is some dispersion in measured pore sizes, these structures can best be described as having a uniform pore size.