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Ultra‐thin layer chromatography with integrated silver colloid‐based SERS detection
Author(s) -
Wallace Ryan A.,
Lavrik Nickolay V.,
Sepaniak Michael J.
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600319
Subject(s) - colloid , biomolecule , fluorescence , chemistry , analyte , surface enhanced raman spectroscopy , nanotechnology , hypoxanthine , sulforhodamine b , raman spectroscopy , combinatorial chemistry , materials science , chromatography , organic chemistry , raman scattering , biochemistry , physics , quantum mechanics , cytotoxicity , optics , in vitro , enzyme
Simplified lab‐on‐a‐chip techniques are desirable for quick and efficient detection of analytes of interest in the field. The following work involves the use of deterministic pillar arrays on the micro‐scale as a platform to separate compounds, and the use of Ag colloid within the arrays as a source of increased signal via surface enhanced Raman spectroscopy (SERS). One problem traditionally seen with SERS surfaces containing Ag colloid is oxidation; however, our platforms are superhydrophobic, reducing the amount of oxidation taking place on the surface of the Ag colloid. This work includes the successful separation and SERS detection of a fluorescent dye compounds (resorufin and sulforhodamine 640), fluorescent anti‐tumor drugs (Adriamycin and Daunomycin), and purine and pyrimidine bases (adenine, cytosine, guanine, hypoxanthine, and thymine).