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Charge‐based separation of particles and cells with similar sizes via the wall‐induced electrical lift
Author(s) -
Thomas Cory,
Lu Xinyu,
Todd Andrew,
Raval Yash,
Tzeng TzuenRong,
Song Yongxin,
Wang Junsheng,
Li Dongqing,
Xuan Xiangchun
Publication year - 2017
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600284
Subject(s) - electrokinetic phenomena , polystyrene , materials science , microchannel , electric field , particle (ecology) , lift (data mining) , mechanics , analytical chemistry (journal) , nanotechnology , chromatography , chemistry , polymer , composite material , physics , oceanography , quantum mechanics , geology , computer science , data mining
The separation of particles and cells in a uniform mixture has been extensively studied as a necessity in many chemical and biomedical engineering and research fields. This work demonstrates a continuous charge‐based separation of fluorescent and plain spherical polystyrene particles with comparable sizes in a ψ‐shaped microchannel via the wall‐induced electrical lift. The effects of both the direct current electric field in the main‐branch and the electric field ratio in between the inlet branches for sheath fluid and particle mixture are investigated on this electrokinetic particle separation. A Lagrangian tracking method based theoretical model is also developed to understand the particle transport in the microchannel and simulate the parametric effects on particle separation. Moreover, the demonstrated charge‐based separation is applied to a mixture of yeast cells and polystyrene particles with similar sizes. Good separation efficiency and purity are achieved for both the cells and the particles.