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On the electromigration of charged fluorophore‐labeled oligosaccharides in polyethylene oxide solutions
Author(s) -
Jarvas Gabor,
Kerekgyarto Marta,
Guttman András
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600183
Subject(s) - fluorophore , chemistry , arrhenius equation , activation energy , polymer , oxide , matrix (chemical analysis) , diffusion , analyte , electromigration , fluorescence , analytical chemistry (journal) , chemical physics , chromatography , materials science , thermodynamics , organic chemistry , physics , quantum mechanics , composite material
The separation mechanism of charged fluorophore (aminopyrenetrisulfonate)‐labeled maltooligosaccharides with α1‐4 linkages was studied in polyethylene oxide (PEO) solutions (MW 300 000 Da) with special interest to possible analyte and/or network deformations as well as potential solute–matrix interactions. The electrophoretic mobilities of the 8‐aminopyrene‐1,3,6‐trisulfonate‐labeled maltooligosaccharides were found proportional with their MW −2/3 . The Arrhenius function was used to determine the activation energy needed by the labeled sugars to migrate through the separation media. With increasing solute size, the activation energy ( E a ) values decreased in polymer concentrations above the entanglement threshold of the PEO, while showed apparently independent function at the entanglement threshold. The observed phenomenon was considered as a result of solute–matrix interaction, which could be alleviated by the addition of an organic modifier to the BGE.