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Developing a fluorescence‐coupled capillary electrophoresis based method to probe interactions between QDs and colorectal cancer targeting peptides
Author(s) -
Liu Feifei,
Wang Jianhao,
Yang Li,
Liu Li,
Ding Shumin,
Fu Minli,
Deng Linhong,
Gao Liqian
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600165
Subject(s) - capillary electrophoresis , colorectal cancer , fluorescence , peptide , quantum dot , cancer cell , cancer , chemistry , capillary action , cancer detection , biophysics , nanotechnology , cancer research , combinatorial chemistry , chromatography , materials science , biochemistry , biology , medicine , physics , quantum mechanics , composite material
As is well known, quantum dots (QDs) have become valuable probes for cancer imaging. In particular, QD‐labeled targeting peptides are capable of identifying cancer or tumors cells. A new colorectal cancer targeting peptide, cyclo(1, 9)‐CTPSPFSHC, has strong targeting ability and also shows great potential in the identification and treatment of colon cancer. Herein, we synthesized a dual functional polypeptide, cyclo(1, 9)‐CTPSPFSHCD 2 G 2 DP 9 G 3 H 6 (H 6 ‐TCP), to investigate its interaction with QDs inside the capillary. Fluorescence‐coupled CE was adopted and applied to characterize the self‐assembly of H 6 ‐TCP onto QDs. It was indicated that the formation of the assembly was affected by H 6 ‐TCP/QD molar ratio and sampling time. This novel in‐capillary assay greatly reduced the sample consumption and the detection time, which was beneficial for the environment. It is expected that this kind of detection method could find more applications to provide more useful information for cancer diagnosis and detection of harm and hazardous substances/organisms in the environment in the future.