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Enantioselective determination of cathinone derivatives in human hair by capillary electrophoresis combined in‐line with solid‐phase extraction
Author(s) -
Baciu Tatiana,
Borrull Francesc,
Calull Marta,
Aguilar Carme
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600149
Subject(s) - chromatography , cathinone , chemistry , metabolite , solid phase extraction , enantiomer , extraction (chemistry) , capillary electrophoresis , stereochemistry , biochemistry , amphetamine , neuroscience , dopamine , biology
A suitable method has been developed and validated for the chiral separation and determination of R , S ‐mephedrone and one of its metabolites, R , S ‐4‐methylephedrine, and R , S ‐methylenedioxypyrovalerone ( R , S‐ MDPV) in human hair samples by the in‐line coupling between SPE and CD‐assisted CE with a previous sample pretreatment procedure based on pressurized liquid extraction. Optimum separation was achieved on a fused silica‐capillary of 50 μm id and 80 cm total length using 12 mg/mL β‐CD in an aqueous solution of 80 mM disodium phosphate at pH 2.5 as the BGE and an applied voltage of 30 kV. The SPE‐CE device consists of a short length of a capillary of 2 mm packed with Oasis HLB sorbent, which was inserted near to the inlet end of the CE capillary. Several parameters affecting the in‐line preconcentration were evaluated. The LOQs reached for hair samples were 0.05 ng/mg for the enantiomers of mephedrone and its metabolite, and 0.40 ng/mg for the enantiomers of MDPV. The RSDs (%) obtained in intra‐ and interday studies were less than 10% and the relative recoveries were greater than 80%. The method established in this paper is advantageous for its simplicity, overall analysis time and ability to provide information of both enantiomers of a chiral drug in hair samples.