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Analysis of isoquinoline alkaloids using chitosan‐assisted liquid–solid extraction followed by microemulsion liquid chromatography employing a sub‐2‐micron particle stationary phase
Author(s) -
Ye LiHong,
Liu XiDe,
Cao Jun,
Chang Yanxu,
An Mingrui,
Wang ShuLing,
Xu JingJing,
Peng LiQing
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600114
Subject(s) - chromatography , extraction (chemistry) , microemulsion , isoquinoline , acetic acid , chemistry , ethyl acetate , chitosan , solvent , calibration curve , detection limit , particle size , derivative (finance) , organic chemistry , pulmonary surfactant , biochemistry , financial economics , economics
A simple, efficient, and green chitosan‐assisted liquid–solid extraction method was developed for the sample preparation of isoquinoline derivative alkaloids followed by microemulsion LC. The optimized mobile phase consisted of 0.8% w/v of ethyl acetate, 1.0% w/v of SDS, 8.0% w/v of n ‐butanol, 0.1% v/v acetic acid, and 10% v/v ACN. Compared to pharmacopoeia method and organic solvent extraction, this new approach avoided the use of volatile organic solvents, replacing them with relatively small amounts of chitosan. Under the optimum conditions, good linearity ( r 2 > 0.9980) for all calibration curves and low detection limits between 0.05 and 0.10 μg/mL were achieved. The presented procedure was successfully applied to determine alkaloids in Rhizoma coptidis with satisfactory recoveries (81.3–106.4%).