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Microemulsion electrokinetic chromatography as a suitable tool for lipophilicity determination of acidic, neutral, and basic compounds
Author(s) -
Subirats Xavier,
Yuan HuiPing,
Chaves Verónica,
Marzal Núria,
Rosés Martí
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600080
Subject(s) - lipophilicity , microemulsion , chemistry , partition coefficient , electrokinetic phenomena , chromatography , octanol , solvation , micellar electrokinetic chromatography , aqueous solution , acetonitrile , pulmonary surfactant , analytical chemistry (journal) , electrophoresis , organic chemistry , molecule , biochemistry
In the present work, several MEEKC systems are studied to assess their suitability for lipophilicity determination of acidic, neutral, and basic compounds. Thus, several microemulsion compositions over a wide range of pH values (from 2.0 to 12.0), containing heptane, 1−butanol and different types and amounts of surfactant (SDS or sodium cholate: from 1.3 to 3.3%) are characterized using Abraham's solvation model. The addition of acetonitrile (up to 10%) is also studied, since it increases the resolution of the technique for the most lipophilic compounds. The system coefficients obtained are very similar to those of the 1−octanol/water, used as the reference lipophilicity index, allowing simple and linear correlations between the 1−octanol/water partition values (log P o/w ) and MEEKC mass distribution ratios (log k MEEKC ). Variations in the microemulsion composition (aqueous buffer, surfactant, concentration of ACN) did not significantly affect the similarity of the MEEKC systems to log P o/w partition.