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Optimization of microwave‐assisted extraction and pressurized liquid extraction of phenolic compounds from Moringa oleifera leaves by multiresponse surface methodology
Author(s) -
RodríguezPérez Celia,
GilbertLópez Bienvenida,
Mendiola Jose Antonio,
QuirantesPiné Rosa,
SeguraCarretero Antonio,
Ibáñez Elena
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600071
Subject(s) - moringa , kaempferol , trolox , extraction (chemistry) , chemistry , response surface methodology , quercetin , dpph , chromatography , flavonoid , trolox equivalent antioxidant capacity , yield (engineering) , antioxidant , food science , organic chemistry , materials science , metallurgy
This work aims at studying the optimization of microwave‐assisted extraction (MAE) and pressurized liquid extraction (PLE) by multi‐response surface methodology (RSM) to test their efficiency towards the extraction of phenolic compounds from Moringa oleifera ( M. oleifera ) leaves. The extraction yield, total phenolic content (TPC), total flavonoid content (TF), DPPH scavenging method and trolox equivalent antioxidant capacity (TEAC) assay were considered as response variables while effects of extraction time, percentage of ethanol, and temperature were studied. Extraction time of 20 min, 42% ethanol and 158°C were the MAE optimum conditions for achieving extraction yield of 26 ± 2%, EC 50 15 ± 2 μg/mL, 16 ± 1 Eq Trolox/100 g dry leaf, 5.2 ± 0.5 mg Eq quercetin/g dry leaf, and 86 ± 4 mg GAE/g dry leaf. Regarding PLE, the optimum conditions that allowed extraction yield of 56 ± 2%, EC 50 21 ± 3 μg/mL, 12 ± 2 mmol Eq Trolox/100 g dry leaf, 6.5 ± 0.2 mg Eq quercetin/g dry leaf, and 59 ± 6 mg GAE/g dry leaf were 128°C, 35% of ethanol, and 20 min. PLE enabled the extraction of phenolic compounds with a higher number of hydroxyl‐type substituents such as kaempferol diglycoside and its acetyl derivatives and those that are sensitive to high temperatures (glucosinolates or amino acids) while MAE allowed better recoveries of kaempferol, quercetin, and their glucosides derivatives.