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Capillary electrophoretic focusing of covalently derivatized protein induced by surfactant
Author(s) -
Oukacine Farid,
Quirino Joselito P.,
Mesbah Kiarach,
Taverna Myriam
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600030
Subject(s) - covalent bond , pulmonary surfactant , electrophoresis , capillary electrophoresis , chemistry , chromatography , capillary action , biophysics , biochemistry , materials science , biology , organic chemistry , composite material
In this communication, we present a very simple strategy to focus covalently derivatized proteins for high sensitivity CE analysis by LIF detection. We demonstrated that the covalently tagged protein can be focused just by adding SDS at a concentration above the CMC in the derivatized sample. Under specific injection conditions, SDS concentration below the CMC is also sufficient to induce the focusing of the tagged protein. This method allows the quantification and detection of the covalently tagged protein in a narrow zone with an efficiency approaching 220 000 plates/m. Very good linearity was obtained for the ubiquitin in a concentration range of 2–25 μM.