Premium
Enhancing resolution of free‐flow zone electrophoresis via a simple sheath‐flow sample injection
Author(s) -
Yang Ying,
Kong FanZhi,
Liu Ji,
Li JunMin,
Liu XiaoPing,
Li GuoQing,
Wang JuFang,
Xiao Hua,
Fan LiuYin,
Cao ChengXi,
Li Shan
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201600002
Subject(s) - free flow electrophoresis , flow (mathematics) , electrophoresis , analytical chemistry (journal) , chromatography , sample (material) , resolution (logic) , materials science , chemistry , mechanics , physics , computer science , biochemistry , gel electrophoresis of proteins , polyacrylamide gel electrophoresis , artificial intelligence , enzyme
In this work, a simple and novel sheath‐flow sample injection method (SFSIM) is introduced to reduce the band broadening of free‐flow zone electrophoresis separation in newly developed self‐balance free‐flow electrophoresis instrument. A needle injector was placed in the center of the separation inlet, into which the BGE and sample solution were pumped simultaneously. BGE formed sheath flow outside the sample stream, resulting in less band broadening related to hydrodynamics and electrodynamics. Hemoglobin and C‐phycocyanin were successfully separated by the proposed method in contrast to the poor separation of free‐flow electrophoresis with the traditional injection method without sheath flow. About 3.75 times resolution enhancement could be achieved by sheath‐flow sample injection method.