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Photo‐crosslinkable hydrogel‐based 3D microfluidic culture device
Author(s) -
Lee Youlee,
Lee Jong Min,
Bae PanKee,
Chung Il Yup,
Chung Bong Hyun,
Chung Bong Geun
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201570071
Subject(s) - self healing hydrogels , microfluidics , gelatin , materials science , neural stem cell , spheroid , stem cell , biomedical engineering , tissue engineering , 3d cell culture , biophysics , nanotechnology , cell culture , cell , chemistry , polymer chemistry , microbiology and biotechnology , biology , medicine , biochemistry , genetics
Electrophoresis 2015, 36 , 994–1001. DOI: 10.1002/elps.201400465 Neural stem cells are of great important cell sources for neurodegenerative disease and spinal cord injury applications. Although the interaction between the neural stem cells and tumors enables the control of the cell fate, their effect is still not clear. To culture the neural stem cells and tumors at the same time, we developed the photo‐crosslinkable hydrogel‐based 3D microfluidic device. The photo‐crosslinkable gelatin methacrylate (GelMA) polymer was employed as a physical barrier in the 3D microfluidic device, showing that the pore size was inversely proportional to GelMA hydrogel concentrations. It revealed that the morphology of pores in 5 w/v% GelMA hydrogels (34 mm pore size) was elliptical shape, whereas we observed circular‐shaped pores in 25 w/v% GelMA hydrogels (4 mm pore size). We successfully demonstrated the co‐culture of the neural stem cells and tumors in the photo‐crosslinkable hydrogel‐based 3D microfluidic device, indicating that most neural stem cells differentiated into neurons, while tumors were cultured in the collagen gels.