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Graphene oxide‐SiO 2 hybrid nanostructure as coating material for capillary electrochromatography
Author(s) -
Qu Qishu,
Xuan Han,
Zhang Kehua,
Ding Yi,
Xu Qin
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500548
Subject(s) - capillary electrochromatography , nanoparticle , coating , capillary action , electrochromatography , chromatography , graphene , adsorption , materials science , monolithic hplc column , oxide , layer (electronics) , chemistry , chemical engineering , nanotechnology , capillary electrophoresis , high performance liquid chromatography , organic chemistry , composite material , engineering
Graphene oxide (GO) has been considered as a promising stationary phase for chromatographic separation. However, the very strong adsorption of the analytes on the GO surface lead to the severe peak tailing, which in turn resulting in decreased separation performance. In this work, GO and silica nanoparticles hybrid nanostructures (GO/SiO 2 NPs@column) were coated onto the capillary inner wall by passing the mixture of GO and silica sol through the capillary column. The successful of coating of GO/SiO 2 NPs onto the capillary wall was confirmed by SEM and electroosmotic flow mobilities test. By partially covering the GO surface with silica nanoparticles, the peak tailing was decreased greatly while the unique high shape selectivity arises from the surface of remained GO was kept. Consequently, compared with the column modified with GO (GO@column), the column modified with GO and silica nanoparticles through layer‐by‐layer method (GO‐SiO 2 NPs@column), or the column modified with silica nanoparticles (SiO 2 NPs@column), GO/SiO 2 NPs@column possessed highest resolutions. The GO/SiO 2 NPs@column was applied to separate egg white and both acidic and basic proteins as well as three glycoisoforms of ovalbumin were separated in a single run within 36 min. The intra‐day, inter‐day, and column‐to‐column reproducibilities were evaluated by calculating the RSDs of the retention of naphthalene and biphenyl in open‐tubular capillary electrochromatography. The RSD values were found to be less than 7.1%.