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Development of the 19 X‐STR loci multiplex system and genetic analysis of a Zhejiang Han population in China
Author(s) -
Yang XingYi,
Wu WeiWei,
Chen LinLi,
Liu ChangHui,
Zhang XiaoFang,
Chen Ling,
Feng XingLin,
Wang HuiJun,
Liu Chao
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500540
Subject(s) - multiplex , multiplex polymerase chain reaction , str multiplex system , biology , genetics , microsatellite , microbiology and biotechnology , polymerase chain reaction , gene , allele
The 19 X‐STRs multiplex system is a PCR‐based amplification kit that facilitates simultaneous amplification of 19 X‐chromosomal STR loci (i.e. DXS7423, DXS10148, DXS10159, DXS6809, DXS7424, DXS8378, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103,DXS10101, HPTRB, DXS10075, DXS10074, DXS10135, and DXS10134). Eleven loci were extensively used in an Investigator Qiagen Argus X‐12 (DXS7423, DXS10148, DXS8378, DXS10162, DXS7132, DXS10079, DXS10103, DXS10101, HPTRB, DXS10074, and DXS10135). In this research, the multiplex system was tested for detection sensitivity, DNA mixtures, inhibitor tolerance and species specificity; SWGDAM Validation Guidelines – Approved December 2012 were followed for the human fluorescent STR multiplex PCR reagent. Samples from 181 unrelated Zhejiang Han individuals (121 males and 60 females) were typed using this multiplex system. The results show that this 19X‐STRs multiplex system is a robust and reliable amplification means to facilitate forensic and human identification testing.