Premium
The potential of SNP‐based PCR‐RFLP capillary electrophoresis analysis to authenticate and detect admixtures of Mediterranean olive oils
Author(s) -
Bazakos Christos,
Khanfir Emna,
Aoun Mariem,
Spano Thodhoraq,
Zein Zeina El,
Chalak Lamis,
Riachy Milad El,
AbouSleymane Gretta,
Ali Sihem Ben,
Grati Kammoun Naziha,
Kalaitzis Panagiotis
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500537
Subject(s) - olive oil , capillary electrophoresis , context (archaeology) , single nucleotide polymorphism , chromatography , snp , traceability , dna extraction , biology , polymerase chain reaction , computational biology , food science , genetics , chemistry , computer science , gene , genotype , paleontology , software engineering
Authentication and traceability of extra virgin olive oil is a challenging research task due to the complexity of fraudulent practices. In this context, the monovarietal olive oils of Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI) require new tests and cutting edge analytical technologies to detect mislabeling and misleading origin. Toward this direction, DNA‐based technologies could serve as a complementary to the analytical techniques assay. Single nucleotide polymorphisms are ideal molecular markers since they require short PCR analytical targets which are a prerequisite for forensic applications in olive oil sector. In the present study, a small number of polymorphic SNPs were used with an SNP‐based PCR‐RFLP capillary electrophoresis platform to discriminate six out of 13 monovarietal olive oils of Mediterranean origin from three different countries, Greece, Tunisia, and Lebanon. Moreover, the high sensitivity of capillary electrophoresis in combination with the DNA extraction protocol lowered the limit of detection to 10% in an admixture of Tsounati in a Koroneiki olive oil matrix.