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Monoliths in capillary electrochromatography and capillary liquid chromatography in conjunction with mass spectrometry
Author(s) -
Moravcová Dana,
Rantamäki Antti H.,
Duša Filip,
Wiedmer Susanne K.
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500520
Subject(s) - capillary electrochromatography , electrochromatography , chromatography , capillary action , mass spectrometry , monolith , monolithic hplc column , chemistry , analyte , capillary electrophoresis , capillary electrophoresis–mass spectrometry , materials science , high performance liquid chromatography , electrospray ionization , biochemistry , composite material , catalysis
Here, we have reviewed separation studies utilizing monolithic capillary columns for separation of compounds preceding MS analysis. The review is divided in two parts according to the used separation method, namely CEC and capillary LC (cLC). Based on our overview, monolithic CEC‐MS technique have been more focused on the syntheses of highly specialized and selective separation phase materials for fast and efficient separation of specific types of analytes. In contrast, monolithic cLC‐MS is more widely used and is often employed, for instance, in the analysis of oligonucleotides, metabolites, and peptides and proteins in proteomic studies. While poly(styrene‐divinylbenzene)‐based and silica‐based monolithic capillaries found their place in proteomic analyses, the other laboratory‐synthesized monoliths still wait for their wider utilization in routine analyses. The development of new monolithic materials will most likely continue due to the demand of more efficient and rapid separation of increasingly complex samples.