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Capillary electrophoresis combined in‐line with solid‐phase extraction using magnetic particles as new adsorbents for the determination of drugs of abuse in human urine
Author(s) -
Baciu Tatiana,
Borrull Francesc,
Neusüß Christian,
Aguilar Carme,
Calull Marta
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500515
Subject(s) - chromatography , solid phase extraction , sorbent , capillary electrophoresis , urine , extraction (chemistry) , adsorption , magnetic nanoparticles , chemistry , detection limit , materials science , nanoparticle , nanotechnology , biochemistry , organic chemistry
A simple approach is presented based on the in‐line coupling between magnetic particles‐based SPE and CE. Silica‐coated iron oxide particles functionalized with C 18 were successfully synthesized and used as a reverse‐phase sorbent for in‐line SPE‐CE. Magnets were used to locally immobilize these sorbents inside the capillary. Four drugs of abuse were preconcentrated and determined in urine samples using the developed method with a simple pretreatment procedure based on LLE. Several parameters affecting the preconcentration were evaluated. The obtained results show that this strategy enhanced detection sensitivity in the range of 125–700‐fold compared with CE without preconcentration. The developed method provides LODs (S/N = 3) for standard samples in the range of 0.5–20 ng/mL with satisfactory analytical precision, in both intraday and day‐to‐day experiments (RSDs <20%). The LODs (S/N = 3) reached for urine samples were in the range of 20−50 ng/mL. Relative recoveries greater than 75.9% were obtained. The established method has been applied to the analysis of drugs of abuse in urine samples from drug abusers.