Premium
Development of a rapid 21‐plex autosomal STR typing system for forensic applications
Author(s) -
Yang Meng,
Yin Caiyong,
Lv Yuexin,
Yang Yaran,
Chen Jing,
Yu Zailiang,
Liu Xu,
Xu Meibo,
Chen Feng,
Wu Huijuan,
Yan Jiangwei
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500498
Subject(s) - forensic science , typing , computational biology , forensic genetics , biology , genetics , str multiplex system , microsatellite , gene , allele
DNA‐STR genotyping technology has been widely used in forensic investigations. Even with such success, there is a great need to reduce the analysis time. In this study, we established a new rapid 21‐plex STR typing system, including 13 CODIS loci, Penta D, Penta E, D12S391, D2S1338, D6S1043, D19S433, D2S441 and Amelogenin loci. This system could shorten the amplification time to a minimum of 90 min and does not require DNA extraction from the samples. Validation of the typing system complied with the Scientific Working Group on DNA Analysis Methods (SWGDAM) and the Chinese National Standard (GA/T815‐2009) guidelines. The results demonstrated that this 21‐plex STR typing system was a valuable tool for rapid criminal investigation.