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Quantitative analysis of serotonin secreted by human embryonic stem cells‐derived serotonergic neurons via pH‐mediated online stacking‐CE‐ESI‐MRM
Author(s) -
Zhong Xuefei,
Hao Ling,
Lu Jianfeng,
Ye Hui,
Zhang SuChun,
Li Lingjun
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500496
Subject(s) - serotonergic , serotonin , chemistry , embryonic stem cell , stacking , chromatography , biochemistry , receptor , organic chemistry , gene
A CE‐ESI‐MRM‐based assay was developed for targeted analysis of serotonin released by human embryonic stem cells‐derived serotonergic neurons in a chemically defined environment. A discontinuous electrolyte system was optimized for pH‐mediated online stacking of serotonin. Combining with a liquid–liquid extraction procedure, LOD of serotonin in the Krebs'‐Ringer's solution by CE‐ESI‐MS/MS on a 3D ion trap MS was0.15 ng/mL. The quantitative results confirmed the serotonergic identity of the in vitro developed neurons and the capacity of these neurons to release serotonin in response to stimulus.