z-logo
Premium
Quantitative analysis of serotonin secreted by human embryonic stem cells‐derived serotonergic neurons via pH‐mediated online stacking‐CE‐ESI‐MRM
Author(s) -
Zhong Xuefei,
Hao Ling,
Lu Jianfeng,
Ye Hui,
Zhang SuChun,
Li Lingjun
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500496
Subject(s) - serotonergic , serotonin , chemistry , embryonic stem cell , stacking , chromatography , biochemistry , receptor , organic chemistry , gene
A CE‐ESI‐MRM‐based assay was developed for targeted analysis of serotonin released by human embryonic stem cells‐derived serotonergic neurons in a chemically defined environment. A discontinuous electrolyte system was optimized for pH‐mediated online stacking of serotonin. Combining with a liquid–liquid extraction procedure, LOD of serotonin in the Krebs'‐Ringer's solution by CE‐ESI‐MS/MS on a 3D ion trap MS was0.15 ng/mL. The quantitative results confirmed the serotonergic identity of the in vitro developed neurons and the capacity of these neurons to release serotonin in response to stimulus.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here