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Fast and simple MEKC sweeping method for determination of thiosulfate in urine
Author(s) -
Kubalczyk Paweł,
Chwatko Grażyna,
Głowacki Rafał
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500411
Subject(s) - thiosulfate , chromatography , chemistry , detection limit , micellar electrokinetic chromatography , sodium thiosulfate , derivatization , calibration curve , reagent , acetonitrile , analytical chemistry (journal) , high performance liquid chromatography , sulfur , inorganic chemistry , organic chemistry
A new method for determination of thiosulfate in human urine has been developed and validated. Analytical procedure is very simple and consists of only few steps: derivatization of thiosulfate with 2‐chloro‐1‐methylquinolinium tetrafluoroborate, centrifugation of a mixture, separation of so‐formed derivative by micellar electrokinetic chromatography with sweeping and UV detection at 375 nm. A fused‐silica capillary with an inlet to detector length of 51.5 cm and a total length of 60 cm (75 μm id) was served as a separation column. The separation voltage of 20.5 kV (∼160 mA) and buffer solution consisting of 0.055 mol/L sodium phosphate (pH 8), 25% acetonitrile, and 0.035 mol/L sodium dodecyl sulfate were found to be the most suitable conditions for the effective separation. The limit of quantification for thiosulfate was 4 μmol/L urine. The method was validated and calibrated for thiosulfate in the range of 4–64 μmol/L (R 2 = 0.9997). The relative standard deviation of the points of the calibration curve varied from 1.2 to 4.8% RSD.