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Activation energy associated with the electromigration of oligosaccharides through viscosity modifier and polymeric additive containing background electrolytes
Author(s) -
Kerékgyártó Márta,
Járvás Gábor,
Novák Levente,
Guttman András
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500394
Subject(s) - activation energy , ethylene glycol , arrhenius equation , polyacrylamide , viscosity , chemistry , intrinsic viscosity , electrolyte , electrophoresis , polymer , analytical chemistry (journal) , polymer chemistry , materials science , organic chemistry , chromatography , electrode , composite material
The activation energy related to the electromigration of oligosaccharides can be determined from their measured electrophoretic mobilities at different temperatures. The effects of a viscosity modifier (ethylene glycol) and a polymeric additive (linear polyacrylamide) on the electrophoretic mobility of linear sugar oligomers with α1–4 linked glucose units (maltooligosaccharides) were studied in CE using the activation energy concept. The electrophoretic separations of 8‐aminopyrene‐1,3,6‐trisulfonate‐labeled maltooligosaccharides were monitored by LIF detection in the temperature range of 20–50°C, using either 0–60% ethylene glycol (viscosity modifier) or 0–3% linear polyacrylamide (polymeric additive) containing BGEs. Activation energy curves were constructed based on the slopes of the Arrhenius plots. With the use of linear polyacrylamide additive, solute size‐dependent activation energy variations were found for the maltooligosaccharides with polymerization degrees below and above maltoheptaose (DP 7), probably due to molecular conformation changes and possible matrix interaction effects.