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Far infrared‐assisted encapsulation of filter paper strips in poly(methyl methacrylate) for proteolysis
Author(s) -
Chen Qiwen,
Bao Huimin,
Zhang Luyan,
Chen Gang
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500358
Subject(s) - proteolysis , encapsulation (networking) , strips , methyl methacrylate , materials science , infrared , filter paper , methacrylate , chemistry , copolymer , chromatography , polymer , optics , organic chemistry , composite material , computer science , physics , computer network , enzyme
Filter paper strips were enclosed between two poly(methyl methacrylate) plates to fabricate paper‐packed channel microchips under pressure in the presence of far infrared irradiation. After the enclosed paper strip was oxidized by periodate, trypsin was covalently immobilized in them to fabricate microfluidic proteolysis bioreactor. The feasibility and performance of the unique bioreactor were demonstrated by digesting BSA and lysozyme. The results were comparable to those of conventional in‐solution proteolysis while the digestion time was significantly reduced to ∼18 s. The suitability of the microfluidic paper‐based bioreactors to complex proteins was demonstrated by digesting human serum.