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Capillary electrochromatography and nano‐liquid chromatography coupled to nano‐electrospray ionization interface for the separation and identification of estrogenic compounds
Author(s) -
D'Orazio Giovanni,
HernándezBorges Javier,
AsensioRamos María,
RodríguezDelgado Miguel Ángel,
Fanali Salvatore
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500327
Subject(s) - chromatography , capillary electrochromatography , chemistry , electrospray , electrochromatography , nano , resolution (logic) , analytical chemistry (journal) , electrospray ionization , selectivity , mass spectrometry , capillary electrophoresis , materials science , organic chemistry , artificial intelligence , computer science , composite material , catalysis
Nano‐LC and CEC were coupled to MS through a nanospray or a pressurized liquid‐junction interface for the simultaneous separation and determination of 11 estrogenic compounds. Different stationary phases, that is, phenyl, C18, and C18 bidentate silica hydrate, were studied. For both techniques, the phenyl stationary phase was the best option, considering separation efficiency, selectivity, and resolution. Under the optimized conditions, the baseline separation of the target compounds (including estradiol and zearalanol epimers) was achieved in less than 20 min in nano‐LC‐MS and less than 13 min in CEC‐MS. Molecular imprinted polymer SPE was used for extracting the target compounds from mineral water samples with the analysis of nano‐LC‐MS. The whole molecular imprinted polymer SPE nano‐LC‐MS method was validated through a recovery study at two levels of concentration. Sensitivity was improved by on‐column focusing technique obtaining LODs in the range 1.4–55.4 ng/L.