Premium
I mproving the sensitivity in chiral capillary electrophoresis
Author(s) -
SánchezLópez Elena,
Marina María Luisa,
Crego Antonio L.
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500315
Subject(s) - capillary electrophoresis , capillary action , sensitivity (control systems) , chromatography , separation (statistics) , reagent , chemistry , capillary electrochromatography , materials science , analytical chemistry (journal) , computer science , machine learning , electronic engineering , engineering , composite material
CE is known for being one of the most powerful analytical techniques when performing enantioseparations due to its numerous advantages such as excellent separation efficiency and extremely low solvents and reagents consumption, all of them derived from the capillary small dimensions. Moreover, it is worth highlighting that unlike in chromatographic techniques, in CE the chiral selector is generally within the separation medium instead of being attached to the separation column which makes the method optimization a more versatile task. Despite its numerous advantages, when using UV‐Vis detection, CE lacks of sensitivity detection due to its short optical path length derived from the narrow separation capillary. This issue can be overcome by means of different approaches, either by sample treatment procedures or by in‐capillary preconcentration techniques or even by employing detection systems more sensitive than UV‐Vis, such as LIF or MS. The present review assembles the latest contributions regarding improvements of sensitivity in chiral CE published from June 2013 until May 2015, which follows the works included in a previous review reported by Sánchez‐Hernández et al. [ Electrophoresis 2014, 35, 12–27].