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Nondenaturing polyacrylamide gel electrophoresis to study the dissociation of the p53·MDM2/X complex by potentially anticancer compounds
Author(s) -
Sgammato Roberta,
Desiderio Doriana,
Lamberti Anna,
Raimo Gennaro,
Novellino Ettore,
Carotenuto Alfonso,
Masullo Mariorosario
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500305
Subject(s) - mdmx , dissociation (chemistry) , chemistry , polyacrylamide gel electrophoresis , mdm2 , small molecule , chromatography , biophysics , gel electrophoresis , apoptosis , combinatorial chemistry , biochemistry , biology , enzyme , organic chemistry
A new analytical method to study the dissociation of the complexes between the oncosuppressor p53 and its negative modulators murine double‐minute protein 2 (MDM2) or MDMX, is proposed. This technique is reliable to determine the dissociative power exerted by small molecules on the complex taking advantage of the appearance of migrating MDM2 or MDMX in a native polyacrylamide gel, when inhibitors are added to the complex mixture. Therefore, we propose this new approach to easily screen library of compounds, with potential pharmacological anticancer activity.