Online probing quantum dots and engineered enzyme self‐assembly in a nanoliter scale | Zendy

Wang Jianhao | Zendy; Li Jinchen | Zendy; Wang Jianpeng | Zendy; Wang Cheli | Zendy; Teng Tiwan | Zendy; Chen Yao | Zendy; Li Jinping | Zendy; Dong Bingyu | Zendy; Qiu Lin | Zendy; Jiang Pengju | Zendy

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Online probing quantum dots and engineered enzyme self‐assembly in a nanoliter scale

Author(s) -

Wang Jianhao,

Li Jinchen,

Wang Jianpeng,

Wang Cheli,

Teng Tiwan,

Chen Yao,

Li Jinping,

Dong Bingyu,

Qiu Lin,

Jiang Pengju

Publication year - 2016

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.201500295

Subject(s) - quantum dot , self assembly , nanotechnology , fluorescence , nanoparticle , materials science , enzyme , scale (ratio) , chemistry , biophysics , physics , biochemistry , biology , quantum mechanics

Nanoparticles provide significantly enhanced binding characteristics. However, fast online probing of the self‐assembly process remains hard to achieve in practice. Herein, we report a fluorescence coupled CE method for probing the self‐assembly events between quantum dots (QDs) and engineered Jumonji domain‐containing protein 6 (Jmjd6) enzyme. QDs and Jmjd6 were sequentially injected into the capillary, where the self‐assembly took place in a nanoliter scale. In particular, we showed that the Jmjd6/QD ratio, the interval time, and the injection volume had a great effect on the online self‐assembly. The current approach may allow for a better understanding of QDs–enzyme self‐assembly and enzymatic activity detection.

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