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Online probing quantum dots and engineered enzyme self‐assembly in a nanoliter scale
Author(s) -
Wang Jianhao,
Li Jinchen,
Wang Jianpeng,
Wang Cheli,
Teng Tiwan,
Chen Yao,
Li Jinping,
Dong Bingyu,
Qiu Lin,
Jiang Pengju
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500295
Subject(s) - quantum dot , self assembly , nanotechnology , fluorescence , nanoparticle , materials science , enzyme , scale (ratio) , chemistry , biophysics , physics , biochemistry , biology , quantum mechanics
Nanoparticles provide significantly enhanced binding characteristics. However, fast online probing of the self‐assembly process remains hard to achieve in practice. Herein, we report a fluorescence coupled CE method for probing the self‐assembly events between quantum dots (QDs) and engineered Jumonji domain‐containing protein 6 (Jmjd6) enzyme. QDs and Jmjd6 were sequentially injected into the capillary, where the self‐assembly took place in a nanoliter scale. In particular, we showed that the Jmjd6/QD ratio, the interval time, and the injection volume had a great effect on the online self‐assembly. The current approach may allow for a better understanding of QDs–enzyme self‐assembly and enzymatic activity detection.