Premium
Recent applications of CE‐ and HPLC‐MS in the analysis of human fluids
Author(s) -
Iadarola Paolo,
Fumagalli Marco,
Bardoni Anna Maria,
Salvini Roberta,
Viglio Simona
Publication year - 2016
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500272
Subject(s) - biological fluids , body fluid , amniotic fluid , synovial fluid , exhaled breath condensate , bronchoalveolar lavage , proteomics , computer science , computational biology , chromatography , pathology , medicine , chemistry , biology , lung , asthma , gene , pregnancy , fetus , biochemistry , alternative medicine , genetics , osteoarthritis
The present review intends to cover the literature on the use of CE‐/LC‐MS for the analysis of human fluids, from 2010 until present. It has been planned to provide an overview of the most recent practical applications of these techniques to less extensively used human body fluids, including, bronchoalveolar lavage fluid, synovial fluid, nipple aspirate, tear fluid, breast fluid, amniotic fluid, and cerumen. Potential pitfalls related to fluid collection and sample preparation, with particular attention to sample clean‐up procedures, and methods of analysis, from the research laboratory to a clinical setting will also be addressed. While being apparent that proteomics/metabolomics represent the most prominent approaches for global identification/quantification of putative biomarkers for a variety of human diseases, evidence is also provided of the suitability of these sophisticated techniques for the detection of heterogeneous components carried by these fluids.