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A simple and compact fluorescence detection system for capillary electrophoresis and its application to food analysis
Author(s) -
Zhai Haiyun,
Yuan Kaisong,
Yu Xiao,
Chen Zuanguang,
Liu Zhenping,
Su Zihao
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500265
Subject(s) - rhodamine 6g , fluorescence , detection limit , photomultiplier , optical fiber , capillary electrophoresis , capillary action , materials science , laser induced fluorescence , optics , optoelectronics , optical filter , rhodamine , laser , chromatography , chemistry , physics , detector , composite material
A novel fluorescence detection system for CE was described and evaluated. Two miniature laser pointers were used as the excitation source. A Y‐style optical fiber was used to transmit the excitation light and a four‐branch optical fiber was used to collect the fluorescence. The optical fiber and optical filter were imported into a photomultiplier tube without any extra fixing device. A simplified PDMS detection cell was designed with guide channels through which the optical fibers were easily aligned to the detection window of separation capillary. According to different requirements, laser pointers and different filters were selected by simple switching and replacement. The fluorescence from four different directions was collected at the same detecting point. Thus, the sensitivity was enhanced without peak broadening. The fluorescence detection system was simple, compact, low‐cost, and highly sensitive, with its functionality demonstrated by the separation and determination of red dyes and fluorescent whitening agents. The detection limit of rhodamine 6G was 7.7 nM (S/N = 3). The system was further applied to determine illegal food dyes. The CE system is potentially eligible for food safety analysis.