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Micellar electrokinetic chromatography with short‐end injection for rapid separation and simultaneous determination of aesculin, aesculetin, and phenylephrine in pharmaceutical preparations
Author(s) -
Pincová Lucie,
Petrů Klára,
Pospíšilová Marie,
Polášek Miroslav
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500215
Subject(s) - chromatography , chemistry , calibration curve , micellar electrokinetic chromatography , electrokinetic phenomena , capillary electrophoresis , detection limit
A fast micellar electrokinetic chromatography (MEKC) method for simultaneous assay of aesculin, aesculetin, and phenylephrine was developed and validated. The separation was carried out in a fused‐silica capillary (50 μm id, total length 64.5 cm, effective length 8.5 cm) with UV detection at 210 nm, temperature 25°C and separation voltage –25 kV. The samples were loaded hydrodynamically at a pressure of –50 mbar for 6 s. The background electrolyte of pH 8.6 contained 20 mM boric acid, 60 mM SDS, and 5% (v/v) of methanol. The calibration curves were linear in the range 10−500 μg/mL for aesculin and aesculetin and 12.5–625 μg/mL for phenylephrine. The RSD values of corrected peak areas were 0.6−1.2% ( n = 6) when determining 0.2 mg/mL of aesculin and aesculetin and 0.25 mg/mL of phenylephrine in prepared standard mixtures. The method was successfully applied to the assay of aesculin and phenylephrine in a pharmaceutical preparation (RSD = 1.9−2.0%; n = 3) and the robustness of the method for both, the determination of analytes and the system suitability test parameter values, was evaluated with the use of Plackett‐Burman design.