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Combination of two‐dimensional gel electrophoresis and a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue allows the identification of intracellular cisplatin–protein adducts
Author(s) -
Kotz Sandra,
Kullmann Maximilian,
Crone Barbara,
Kalayda Ganna V.,
Jaehde Ulrich,
Metzger Sabine
Publication year - 2015
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201500188
Subject(s) - cisplatin , intracellular , adduct , chemistry , fluorescence , biochemistry , pharmacology , chemotherapy , biology , genetics , physics , organic chemistry , quantum mechanics
Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin–protein adducts was developed using a fluorescent carboxyfluorescein‐diacetate‐labeled cisplatin analogue (CFDA–cisplatin), 2DE, and ESI‐MS/MS. We identified several CFDA–cisplatin–protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA–cisplatin–protein adducts, which may help to understand the resistance mechanism of cisplatin.